1. Instrumentation in Plant tissue culture
i. Hot air oven
ii. Autoclave unit
iii. Culture vessels and racks
iv. Incubation chamber
v. Rotary Shaker
vi. Deep freezer
vii. Laminar air flow unit

2. Sterilization technique:
i. Glassware
ii. Explant preparation
iii. Standard protocol for tissue culture

3. Preparation of culture media:
i. Preparation of stock solutions of MS media
ii. Preparation of MS media.

4. Callus culture:
i. Embryo culture
ii. Anther culture

5. Isolation and culture of plant protoplast
i. Protoplast isolation by mechanical method
ii. Protoplast culture using microchamber method
iii. Protoplast culture using suspension culture technique.