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Module of Environmental Biotechnology (Duration – 30 days)
Module of Environmental Biotechnology (Duration – 30 days)
  • INTRODUCTORY LECTURE BY LCD PRESENTATION
  • DEMONSTRATION OF THE INSTRUMENTS
    • BOD Incubator
    • UV –Vis Spectrophotometer
    • Automated cell counter
    • Laminar Air flow
    • Autoclave
    • Conductivity meter
    • Water Bath
    • Thermometer
    • Microscope
    • pH meter
    • Colony Counter
    • Hot air oven
    • Centrifuge
    • Digital balance
  • PRACTICAL EXERCISES
  • STUDY OF SOME IMPORTANT WATER QUALITY PARAMETERS(PHYSICAL)
    • Conductivity
    • Temperature
    • Colour
    • Odour
    • Turbidity
  • CHEMICAL ANALYSIS OF WATER
    • Dissolved Oxygen
    • BOD
    • COD
    • pH
    • Hardness
  • BIOLOGICAL ANALYSIS OF WATER (MICROBIOLOGICAL EXAMINATION)
  1. Sterilization and disinfection
    • Dry heat sterilization
    • Moist heat sterilization
  2. Preparation of nutrient agar
    • Weighing of Chemicals by Digital Balance
    • Dissolving of chemicals
    • pH determination by Digital pH meter
    • Addition of solidifying agent Agar-Agar
    • Autoclaving
  • Standard Plate Count (SPC)
    • Sampling
    • Preparation of Nutrient Agar Medium
    • Inoculation
    • Incubation
    • Colony Counting
  • MNP of Coliforms:
    • Sampling
    • Preparation of McConkey’s Broth
    • Durham’s Vial Test
    • Incubation
  • Detection of Salmonella
    • Concentration of Enrichment
    • Prepartion of Tetrathionate Broth
    • Isolation