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Module of Medical Microbiology Duration – 30 days)
Module of Medical Microbiology Duration – 30 days)

(Module includes LCD presentation, Demonstration of instruments, practical exercise, Clinical laboratory working experience & personality development) 

1. INTRODUCTORY LECTURE BY LCD PRESENTATION

2. DEMONSTRATION OF THE INSTRUMENTS
    · Laminar Air flow
    · Autoclave
    · Microscope
    · pH meter
    · Colony Counter
    · Hot air oven
    · Thermal Cycler
    · ELISA reader
    · Automated cell counter
    · Centrifuge
    · Incubator
    · Digital balance
    · BacT/ ALERT

3. PRACTICAL EXERCISES
A. Basic Techniques
a) Sterilization and disinfection
    · Dry heat sterilization
    · Moist heat sterilization
b) Preparation of culture media
    · Weighing of Chemicals by Digital Balance
    · Dissolving of chemicals 
    · pH determination by Digital pH meter
    · Addition of solidifying agent Agar-Agar
    · Autoclaving 
B. ISOLATION AND IDENTIFICATION OF PATHOGENS FROM :
    · Genital Urinary tract
    · Lower and upper respiratory tract
    · Exudates
    · Body fluids (CSF, Plural, Pericardial, Peritoneal)
    · Blood culture by Rapid Method (BacT/AlerT)
    · Others
D. ISOLATION TECHNIQUE
    · Spread plate
    · Streak plate
    · Pour plate / serial dilution
C. SEROLOGY
    · VDRL
    · WIDAL
D. ISOLATION TECHNIQUE
    · Spread plate
    · Streak plate
    · Pour plate / serial dilution
E. IDENTIFICATION OF ORGANISMS
a) Colony Morphology
b) Staining methods
    · Simple staining
    · Gram staining
    · Negative staining
F. STUDY OF PARASITES 
    · Stool examination for various ova & cyst 
    · Plasmodium species (Malaria parasite)
G. ISOLATION AND IDENTIFICATION OF FUNGI
    · KOH staining
    · Lectophenol staining
    · Culture on SDA (Sabourade Dextrose Agar)