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Module of Environmental Biotechnology (Duration – 60 days)
Module of Environmental Biotechnology (Duration – 60 days)
1. INTRODUCTORY LECTURE BY LCD PRESENTATION 
2. DEMONSTRATION OF THE INSTRUMENTS
  • BOD Incubator
  • UV –Vis Spectrophotometer
  • Automated cell counter
  • Laminar Air flow
  • Autoclave
  • Conductivity meter
  • Water Bath
  • Thermometer
  • Microscope
  • pH meter
  • Colony Counter
  • Hot air oven
  • Centrifuge
  • Digital balance

3. PLANT BIOASSAYS:

  • Estimation  of Chlorophyll and Carotenoids
  • Estimation of carbohydrates
  • Determination of nitrogen

4. STUDY OF SOME IMPORTANT WATER QUALITY PARAMETERS:

          A. PHYSICAL ANALYSIS OF WATER
    • Conductivity
    • Temperature
    • Colour
    • Odour
    • Turbidity

    B.   CHEMICAL ANALYSIS OF WATER
    • Dissolved Oxygen
    • BOD
    • COD
    • pH
    • Hardness
    • TDS
    • Chloride
    • Calcium Carbonate

C.   BIOLOGICAL ANALYSIS OF WATER (MICROBIOLOGICAL EXAMINATION)

  • Standard Plate Count (SPC)
    • Sampling
    • Preparation of Nutrient Agar Medium
    • Inoculation
    • Incubation
    • Colony Counting
  • MNP of Coliforms:
    • Sampling
    • Preparation of McConkey’s Broth
    • Durham’s Vial Test
    • Incubation
  • Detection of Salmonella
    • Concentration of Enrichment
    • Prepartion of Tetrathionate Broth
    • Isolation

5. STUDY OF ECOLOGICAL ADAPTATIONS:

  • Ecological adaptations in hydrophytes
  • Ecological adaptations in xerophytes