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Module of Immunotechnology (Duration – 60 days)

(Module includes LCD presentation, Demonstration of instruments, practical exercise, Clinical laboratory working experience & personality development) 

1. INTRODUCTORY LECTURE BY LCD PRESENTATION

2. DEMONSTRATION OF THE INSTRUMENTS
    · Laminar Air flow
    · Autoclave
    · Microscope
    · pH meter
    · Colony Counter
    · Hot air oven
    · Thermal Cycler
    · ELISA reader
    · Automated cell counter
    · Centrifuge
    · Incubator
    · Digital balance
    · Advia Centaur
    · Immulite
    · Electrophoresis unit
    · RIA Counter

3. PRACTICAL EXERCISES

A. GEL DIFFUSION 

    · Ouchterlony Double diffusion (ODD)
    · Radial Immuno Diffusion (RID)

B. AGGLUTINATION REACTION 
    · Blood grouping and Rh typing
    · VDRL (Rapid plasma regain test)
    · Widal Test (Tube Agglutination)

C. IMMUNO TURBIDITY

D. ELISA(ENZYME LINKED IMMUNO SORBENT ASSAY)

    · Microwell
    · Dot ELISA

E. IMMUNO ELECTROPHORESIS
    · CCE (Counter Current Electrophoresis)
    · RIE (Rocket Immuno Electrophoresis) 
F. RIA (RADIO IMMUNO ASSAY)
G. CLIA (CHEMILUMINISCENCE IMMUNO ASSAY)
H. ELFA (ENZYME LINKED FLUROSCENT ASSAY)