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Module of Medical Microbiology (Duration – 60 days)
Module of Medical Microbiology (Duration – 60 days)
(Module includes LCD presentation, Demonstration of instruments, practical exercise, Clinical laboratory working experience & personality development) 

1. INTRODUCTORY LECTURE BY LCD PRESENTATION

2. DEMONSTRATION OF THE INSTRUMENTS
    · Laminar Air flow
    · Autoclave
    · Microscope
    · pH meter
    · Colony Counter
    · Hot air oven
    · Thermal Cycler
    · ELISA reader
    · Automated cell counter
    · Centrifuge
    · Incubator
    · Digital balance
    · BacT/ ALERT

3. PRACTICAL EXERCISES

A. Basic Techniques

a) Sterilization and disinfection
    · Dry heat sterilization
    · Moist heat sterilization
b) Preparation of culture media
    · Weighing of Chemicals by Digital Balance
    · Dissolving of chemicals 
    · pH determination by Digital pH meter
    · Addition of solidifying agent Agar-Agar
    · Autoclaving 

B. STAINING METHODES

    · Simple staining
    · Gram staining 
    · Negative staining
    · Endospore staining 
    · Acid fast staining
C. ISOLATION TECHNIQUE
    · Spread plate
    · Streak plate
    · Pour plate / serial dilution

D. TESTS FOR BACTERIAL IDENTIFICATION
    · TSI test
    · Urease test
    · Citrate test
    · Oxidase test
    · Catalyst test
    · Coagulase test
    · Bile Solubility test
    · Growth and Fermentation on Mannitol Salt Agar
    · Others

E. ANTIBIOTIC SENSITIVITY ASSAY
    · Disc method
    · MIC (Minimum Inhibitory Concentration)

F. ISOLATION AND IDENTIFICATION OF PATHOGENS FROM :
    · Geneto Urinary tract
    · Lower and upper respiratory tract
    · Exudates
    · Body fluids (CSF, Plural, Pericardial, Peritoneal)
    · Blood culture by Rapid Method (BacT/AlerT)
    · Others 

G. STUDY OF PARASITES 
    · Stool examination for various ova & cyst 
    · Plasmodium species (Malaria parasite)

H. ISOLATION AND IDENTIFICATION OF FUNGI
    · KOH staining
    · Lectophenol staining
    · Culture on SDA (Sabourade Dextrose Agar)

URINE ANALYSIS
    · Detection of Urine protein
    · Detection of Urine glucose
    · Detection of Ketone bodies in urine sample

L. HAEMATOLOGY 
    · RBC count
    · WBC count
    · Platelet count
    · Peripheral blood film examination
    · Blood grouping and RH typing
    · Hemoglobin test

M. SEROLOGY
    · VDRL (Venereal Disease Research Laboratory) Test
    · CRP (C-reactive protein) Test
    · ASO (Antistreptolysin O) Test
    · HBsAg (Hepatitis B Surface Antigen) Test
    · HIV (Human Immuno deficiency Virus) Test