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Molecular Biology (Duration – 60 days)
Molecular Biology (Duration – 60 days)

(Module includes LCD presentation, Demonstration of instruments, practical exercise, Clinical laboratory working experience & personality development) 

1. INTRODUCTORY LECTURE BY LCD PRESENTATION

2. DEMONSTRATION OF THE INSTRUMENTS
    · Laminar Air flow
    · Autoclave
    · Microscope
    · pH meter
    · Colony Counter
    · Hot air oven
    · Thermal Cycler
    · Centrifuge
    · Incubator
    · Digital balance
    · Electrophoresis unit
    · Spectrophotometer
    · Incubator Shaker
    · Vortex 

3. PRACTICAL EXERCISES BY CANDIDATES.

A. Isolation of Genomic DNA from prokaryotes
B. Isolation of plant DNA
C. Isolation of Plasmid DNA D. Quantitative and Qualitative analysis of Nucleic acids
E. Agarose Gel Electrophoresis
F. SDS PAGE 
G. Restriction Digestion
H. Ligation
I. PCR Techniques
    · Pre PCR 
    · Amplification
    · Gel Electrophoresis