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1. Instrumentation in Plant tissue culture
i. Hot air oven
ii. Autoclave unit
iii. Culture vessels and racks
iv. Aseptic inoculation chamber
iv. Incubation chamber
v. Rotary Shaker
vi. Deep freezer
vii. Laminar air flow unit
2. Sterilization technique:
i. Glassware
ii. Explant preparation
iii. Standard protocol for tissue culture
iv. Disinfection of aseptic room
v. Types of sterilization.
3. Preparation of culture media:
i. Preparation of stock solutions of MS media
ii. Preparation of hormone stocks solution.
iii. Preparation of MS media
iv. Preparation of other specific media required for various techniques.
4. Callus culture:
i. Initiation and maintenance of callus from the inter nodal explants of stem
explants of any available plant material
ii. Embryo culture
iii. Anther culture
iv. Meristem culture
v. Micropropogation by adventitious bud culture.
vi. Micropropogation by enhanced axillary branching.
5. Isolation and culture of plant protoplast
i. Protoplast isolation by mechanical method
ii. Purification of protoplast by sucrose pad density gradient centrifugation
iii. Protoplast culture using microchamber method.
iv. Protoplast culture using suspension culture technique.
6. Encapsulation of embryo to prepare artificial seeds.
7. Preparation, preservation and maintenance of cell lines.