top-left-corner
logotitle
moody
top-right-corner
Module on PCR Techniques
Module on PCR Techniques

(Duration – 7 days)

(Module includes LCD presentation, Demonstration of instruments and practical exercise)
 

1. INTRODUCTORY LECTURE BY LCD PRESENTATION

2. DEMONSTRATION OF THE INSTRUMENTS
    · Laminar Air flow
    · Autoclave
    · Microscope
    · pH meter
    · Colony Counter
    · Hot air oven
    · Thermal Cycler
    · Centrifuge
    · Incubator
    · Digital balance
    · Electrophoresis unit
    · Spectrophotometer
    · Incubator Shaker


3. PRACTICAL EXERCISES
    A. Isolation of Genomic DNA from prokaryotes
    B. Quantitative and Qualitative analysis of Nucleic acids
    C. Agarose Gel Electrophoresis
    D. PCR Techniques
        · Pre PCR
        · Amplification
        · Gel Electrophoresis