(Duration – 7 days)

(Module includes LCD presentation, Demonstration of instruments and practical exercise)
 

1. Instrumentation in Plant tissue culture
   i. Hot air oven
   ii. Autoclave unit
   iii. Culture vessels and racks
   iv. Incubation chamber
   v. Rotary Shaker
   vi. Deep freezer
   vii. Laminar air flow unit

2. Sterilization technique:
     i. Glassware
     ii. Explant preparation
     iii. Standard protocol for tissue culture

3. Preparation of culture media:
    i. Preparation of stock solutions of MS media
    ii. Preparation of MS media.

4. Explant culture:
    i. Embryo culture
    ii. Anther culture

5. Isolation and culture of plant protoplast
     i. Protoplast isolation by mechanical method
    ii. Protoplast culture using microchamber method